Pimeloyl-acp methyl ester
WebOct 2, 2024 · Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only] Links. Source: COG. Taxonomy: cellular …
Pimeloyl-acp methyl ester
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WebNov 9, 2012 · The methyl ester of this product is then cleaved by BioH to give pimeloyl-ACP which reacts with alanine in the BioF reaction to give the first intermediate of biotin ring assembly. Thus, the methyl ester disguises the biotin synthetic intermediates such that they are accepted as substrates by the fatty acid synthetic pathway [6]. WebOct 8, 2012 · Once pimelate is synthesized, BioH hydrolyzes the methyl ester bond to expose the ω-carboxyl group and terminate chain elongation. The ACP moiety is subsequently released in the BioF reaction, the first …
WebApr 6, 2024 · The pimeloyl-ACP positive control used in our assay was produced in our lab by the demethylation of pimeloyl-ACP methyl ester using the BioJ enzyme (Supplementary Fig. 7b). WebThe pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among ...
WebProtein Pimeloyl-ACP methyl ester carboxylesterase Status UniProtKB unreviewed (TrEMBL) Organism Nakamurella panacisegetis Amino acids 386 Protein existence … WebAug 6, 2024 · We found that this pattern of expression was inversely correlated with transcription of FgbioH1 mRNA, the translated product of which catalyses the hydrolysis of pimeloyl-[ACP] methyl ester to pimeloyl-[ACP], an essential step in …
Webthe methyl group of the seven-carbon pimeloyl-ACP methyl ester is removed by BioH, a short-chain fatty acid esterase, to give pimeloyl-ACP, which is condensed with L-alanine by BioF to start the assembly of the fused rings of biotin (17) (Fig. 1). The E. coli protein of known function most similar to BioC
WebThe enzyme Pimelyl- [acyl-carrier protein] methyl ester esterase (EC 3.1.1.85, BioH; systematic name pimelyl- [acyl-carrier protein] methyl ester hydrolase [1] [2] [3] [4] … new life upholstery ncWebThe malonyl-ACP methyl ester can now enter the fatty acid syn-thetic pathway where it is condensed with malonyl-ACP by a 3-oxoacyl-ACP synthase in a decarboxylating Claisen … into the light once again mangadexWebAug 8, 2010 · Pimeloyl-ACP methyl ester synthesis proceeds either artificially by acylation of ACP with the monomethyl ester of pimelate or physiologically by elongation of a primer … newlife upholsteryWebMay 19, 2024 · Pimeloyl-ACP methyl ester synthesized from P. aeruginosa ACP was also utilized as substrate and gave essentially identical results (data not shown) indicating that the ACP source was... new life upholstery charlotteWebOct 23, 2012 · In Escherichia coli, pimelate is made by bypassing the strict specificity of the fatty acid synthetic pathway. BioC methylates the free carboxyl of a malonyl thioester, which replaces the usual acetyl thioester primer. This atypical primer is transformed to pimeloyl-acyl carrier protein (ACP) methyl ester by two cycles of fatty acid synthesis. newlifeupc normandy tnWebcomplex of BioH with pimeloyl-ACP methyl ester and use the struc-ture to demonstrate that BioH is the gatekeeper and its physio-logical substrate is pimeloyl-ACP methyl ester. cofactor biosynthesis esterase protein–protein interaction Recent work delineated the assembly pathway of the enigmatic pimeloyl moiety of biotin. into the light once again novel spoilerWebVarious nonorthologous substitutes of the bioC -coupled gene bioH from E. coli, observed in several genomes, possibly represent the existence of different pathways for pimeloyl-CoA biosynthesis [4]. Nucleotide sequence of the bioH gene of Escherichia coli [5]. The 104-kDa protein can be converted to the 82-kDa protein upon incubation with Fe3 ... into the light once again marianne